Activity . Take some fruit juice or sugar solution and add some yeast to this. Take this mixture in a test tube fitted with a one-holed cork. Fit the cork with a bent glass tube.
Dip the free end of the glass tube into a test tube containing freshly prepared lime water. What change is observed in the lime water and how long does it take for this change to occur? What does this tell us about the products of fermentation? We have discussed nutrition in organisms in the last section.
The food material taken in during the process of nutrition is used in cells to provide energy for various life processes. Diverse organisms do this in different ways – some use oxygen to break-down glucose completely into carbon dioxide and water, some use other pathways that do not involve oxygen (Fig. . ).
In all cases, the first step is the break-down of glucose, a six-carbon molecule, into a three-carbon molecule called pyruvate. This process takes place in the cytoplasm. Further, the pyruvate may be converted into ethanol and carbon dioxide. This process takes place in yeast during fermentation.
Since this process takes place in the absence of air (oxygen), it is called anaerobic respiration. Break- down of pyruvate using oxygen takes place in the mitochondria. This Figure . Figure .
Figure . Figure . Figure . (a) Air being passed into lime water with a pichkari/ syringe, (b) air being exhaled into lime water process breaks up the three-carbon pyruvate molecule to give three molecules of carbon dioxide.
The other product is water. Since this process takes place in the presence of air (oxygen), it is called aerobic respiration. The release of energy in this aerobic process is a lot greater than in the anaerobic process. Sometimes, when there is a lack of oxygen in our muscle cells, another pathway for the break-down of pyruvate is taken.
Here the pyruvate is converted into lactic acid which is also a three-carbon molecule. This build-up of lactic acid in our muscles during sudden activity causes cramps. Figure . Figure .