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E UKARYOTIC C ELLS · Part 6

Chapter 8: CELL: THE UNIT OF LIFE · BIOLOGY

soluble carotenoid pigments like carotene, xanthophylls and others are present. This gives the part of the plant a yellow, orange or red colour. The leucoplasts are the colourless plastids of varied shapes and sizes with stored nutrients: Amyloplasts store carbohydrates (starch), e.g., potato; elaioplasts store oils and fats whereas Outer membrane Inner membrane Matrix Crista Figure . Structure of mitochondrion (Longitudinal section) Inter-membrane space the aleuroplasts store proteins.

Majority of the chloroplasts of the green plants are found in the mesophyll cells of the leaves. These are lens-shaped, oval, spherical, discoid or even ribbon-like organelles having variable length ( -10µm) and width ( -4µm). Their number varies from per cell of the Chlamydomonas , a green alga to - per cell in the mesophyll. Like mitochondria, the chloroplasts are also double membrane bound.

Of the two, the inner chloroplast membrane is relatively less permeable. The space limited by the inner membrane of the chloroplast is called the stroma. A number of organised flattened membranous sacs called the thylakoids , are present in the stroma (Figure . ).

Thylakoids are arranged in stacks like the piles of coins called grana (singular: granum) or the intergranal thylakoids. In addition, there are flat membranous tubules called the stroma lamellae connecting the thylakoids of the different grana. The membrane of the thylakoids enclose a space called a lumen. The stroma of the chloroplast contains enzymes required for the synthesis of carbohydrates and proteins.

It also contains small, double- stranded circular DNA molecules and ribosomes. Chlorophyll pigments are present in the thylakoids. The ribosomes of the chloroplasts are smaller (70S) than the cytoplasmic ribosomes (80S). .

. Ribosomes Ribosomes are the granular structures first observed under the electron microscope as dense particles by George Palade ( ). They are composed of ribonucleic acid (RNA) and proteins and are not surrounded by any membrane. The eukaryotic ribosomes are 80S while the prokaryotic ribosomes are 70S.

Each ribosome has two subunits, larger and smaller subunits (Fig . ). The two subunits of 80S ribosomes are 60S and 40S while that of 70S ribosomes are 50S and 30S. Here ‘S’ (Svedberg’s Unit)

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