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DNA replication

Chapter 5: CHAPTER 5 · ZOOLOGY · EN medium

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The mode of DNA replication was determined in by Meselson and Stahl. They designed an experiment to distinguish between semi conservative, conservative and dispersive replications. In their experiment, they grew two cultures of E.coli for many generations in separate media. The ‘heavy’ culture was grown in a medium in which the nitrogen source (NH Cl) contained the heavy isotope N and the ‘light’ culture was grown in a medium in which the nitrogen source contained light isotope N for many generations. At the end of growth, they N-DNA Generation I Generation II Gravitational force Heavy Hybrid Light Hybrid N-DNA

📖 Class 12 Zoology English 2024 Edition www.tntextbooks.in · Page 77

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The mode of DNA replication was determined in by Meselson and Stahl. They designed an experiment to distinguish between semi conservative, conservative and dispersive replications. In their experiment, they grew two cultures of E.coli for many generations in separate media. The ‘heavy’ culture was grown in a medium in which the nitrogen source (NH Cl) contained the heavy isotope N and the ‘light’ culture was grown in a medium in which the nitrogen source contained light isotope N for many generations.

At the end of growth, they N-DNA Generation I Generation II Gravitational force Heavy Hybrid Light Hybrid N-DNA

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