ii) Name the hereditary disease for which the first clinical gene therapy was used. iii) Mention the steps involved in gene therapy to treat this disease. . PCR is a useful tool for early diagnosis of an Infectious disease.
Elaborate. . What are recombinant vaccines?. Explain the types.
. Explain why cloning of Dolly, the sheep was such a major scientific breakthrough? . Mention the advantages and disadvantages of cloning.
. Explain how recombinant Insulin can be produced. . What are the possible risks of GMOs?
. ELISA is mainly used for a) Detection of mutations b) Detection of pathogens c) Selecting animals having desired traits d) Selecting plants having desired traits . Transgenic animals are those which have a) Foreign DNA in some of their cells b) Foreign DNA in all their cells c) Foreign RNA in some of their cells d) Foreign RNA in all their cells . Vaccines that use components of a pathogenic organism rather than the whole organism are called a) Subunit recombinant vaccines b) attenuated recombinant vaccines c) DNA vaccines d) conventional vaccines .
GEAC stands for a) Genome Engineering Action Committee b) Ground Environment Action Committee c) Genetic Engineering Approval Committee d) Genetic and Environment Approval Committee . Mention the number of primers required in each cycle of PCR. Write the role of primers and DNA polymerase in PCR. Name the source organism of the DNA polymerase used in PCR.
. How is the amplification of a gene sample of interest carried out using PCR? . What is genetically engineered Insulin?
. Explain how “Rosie” is different from a normal cow. . How was Insulin obtained before the advent of rDNA technology?
What were the problems encountered? . ELISA is a technique based on the principles of antigen-antibody reactions. Can this technique be used in the molecular diagnosis of a genetic disorder such as Phenylketonuria?
. Gene therapy is an attempt to correct a Genetic defect by providing a normal gene XII Std Zoology Chapter XII Std Zoology