of interest is transferred to the host cell through a vector. Consider the following four agents (A to D) in this regard and select correct option about which one or more of these can be used as vector/vectors. [AIPMT MAIN ] A) A bacterium B) Plasmid C) Plasmodium D) Bacteriophage a) (A), (B) and (D) only b) (A) only c) (A) and (C) only d) (B) and (D) only . Which one of the following palindromic base sequences in DNA can be easily cut at about the middle by some particular restriction enzyme?
[AIPMT PRE ] a) ’ – CGTTCG – ’ ’ –ATGGTA - ’ b) ’-GATATG - ’ ’ CTACTA - ’ c) ’ –GAATTC – ’ ’ – CTTAAG- ’ d) ’ –CACGTA - ’ ’ –CTCAGT - ’ . Restriction endonucleases are enzymes which [AIPMT PRE ] a) Make cuts at specific positions within the DNA molecule. b) Recognize a specific nucleotide sequence for binding of DNA ligase. c) Restrict the action of the enzyme DNA polymerase.
d) Remove nucleotides from the ends of the DNA molecule. . Stirred – tank bioreactors have been designed for [AIPMT PRE ] a) Addition of preservatives of the product b) Purification of the product c) Ensuring anaerobic conditions in the culture vessel d) Availability of oxygen throughout the process . There is a retriction endonuclease called EcoRI.
What does ‘co’ part in it stand for? [AIPMT PRE ] a) Coelom b) Coenzyme c) Coli d) Colon . Which one is true state regarding DNA polymerase used in PCR? [AIPMT PRE ] a) It is used to ligate introduced DNA in recipient cells.
b) It serves as selectable marker c) It is isolated from a virus. d) It remains active at high temperature. . For transformation, micro-particles coated with DNA to be bombarded with gene gun are made up of [AIPMT PRE ] a) Silver or Platinum b) Platinum or Zinc c) Silicon or Platinum d) Gold or Tungsten Chapter - Organisms and Population .
Which one of the following is most appropriately defined? [AIPMT MAINS ] a) Host is an organism which provides food