cell anemia, β-thalassemia and phenylketonuria can be detected by PCR in these samples. cDNA from PCR is a valuable tool for diagnosis and monitoring retroviral infections e.g., Corona Virus (SARS - CoV- ). Several virally induced cancers, like cervical cancer caused by Papilloma virus can be detected by PCR. Sex of human beings and live stocks, embryos fertilized invitro can be determined by PCR by using primers and DNA probes specific for sex chromosomes.
PCR technique is also used to detect sex- linked disorders in fertilized embryos. Applications of PCR The differences in the genomes of two different organisms can be studied by PCR. PCR is very important in the study of evolutions, more specifically phylogenetics. PCR Components PCR Process (One Cycle) Nucleotide Denaturation DNA primer Original DNA DNA Sample Primers Nucleotides Taq polymerase Mix Buffer PCR Tubes Thermal Cycler Annealing Extension ’ ’ ’ ’ ’ ’ ’ ’ ’ ’ ’ ’ ’ ’ ’ ’ ’ ’ ’ ’ NA Sample Primers Nucleotides q polymerase Mix Buffer PCR Tubes Th l C l